R2-Pfu Hi-Fi DNA Polymerase
High-fidelity DNA polymerase with 3′→5′ proofreading activity
Key Features
Concentration: 1 U/µL
Fidelity: ~10× higher than Taq (1.6×10⁻⁶/bp/duplication)
Speed: ~30-40 nt/s (0.2-0.4 kb/min) at 72°C
3′→5′ exonuclease activity (proofreading)
Generates blunt-ended products
Required cofactor: Mg²⁺ (2-4 mM MgSO₄)
Optimal temperature: 72°C
High thermostability (greater than Taq at 100°C)
Loading prices...
Product Description
R2-Pfu Hi-Fi DNA Polymerase is a high-fidelity thermostable enzyme derived from the hyperthermophilic archaeon Pyrococcus furiosus. This enzyme is characterized by its exceptional accuracy during DNA synthesis, making it ideal for applications requiring high-fidelity amplification.
It possesses 3′→5′ exonuclease (proofreading) activity, which allows it to correct errors during DNA synthesis, resulting in an error rate approximately 10 times lower than Taq polymerase (1.6×10⁻⁶/bp/duplication).
Concentration: 1 U/µL
Main Advantages
- Superior fidelity: Significantly lower error rate compared to conventional polymerases (~10× higher than Taq)
- Proofreading activity: 3′→5′ exonuclease corrects misincorporated bases
- High thermostability: Greater stability than Taq at elevated temperatures
- Blunt-end cloning: Products ready for blunt-end vector ligation
Origin and Production
R2-Pfu Hi-Fi DNA Polymerase is produced recombinantly in Escherichia coli cells transformed with the cloned pol gene from Pyrococcus furiosus. The enzyme belongs to Family B (α-like) DNA polymerases and is purified to high homogeneity.
Physical Properties
Enzymatic Properties
Extension Rate
~30-40 nt/s (0.2-0.4 kb/min) at 72°C. Slower than Taq, requires ~2 min/kb extension.
Fidelity
1.6×10⁻⁶/bp/duplication (~10× better than Taq)
Required Cofactor
Mg²⁺ (as MgSO₄): 2-4 mM