KOD-on DNA Polymerase
High-fidelity thermostable DNA polymerase with 3′→5′ exonuclease activity for error correction. Processing speed of 1 kb per 15 seconds at optimal temperature (72°C).
Key Features
Concentration: 1 U/μL
Superior fidelity: Significantly lower error rate compared to conventional polymerases
High processivity: Ability to synthesize long DNA fragments without dissociating from the template
Extension speed: 1 kb per 15 seconds at 72°C
3′→5′ exonuclease activity (proofreading)
Optimized performance: Greater product yield in less reaction time
Required cofactor: Mg²⁺ (optimal 2.0-3.0 mM MgSO₄)
Optimal reaction temperature: 68-72°C
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Main Advantages
- Superior fidelity: Significantly lower error rate compared to conventional polymerases
- High processivity: Ability to synthesize long DNA fragments without dissociating from the template
- Optimized performance: Greater product yield in less reaction time
- Versatility: Compatible with multiple applications requiring high precision
Quality Control
All batches are subjected to rigorous testing:
- PCR activity: Verification of amplification capacity
- SDS-PAGE analysis: Confirmation of purity
Storage Conditions
Preservation formulation (1 U/μL):
- Tris-HCl 50 mM
- KCl 50 mM
- DTT 1 mM
- EDTA 0.1 mM
- Glycerol 50%
- Nonidet P-40: 0.1%
- Tween-20: 0.1%
- pH: 8.0
Storage temperature:
-20°C
Note: Avoid repeated freeze-thaw cycles. It is recommended to prepare aliquots for frequent use.