AuTaq DNA Polymerase
Thermostable DNA polymerase from Thermus aquaticus. The gold standard enzyme for routine PCR applications and TA cloning.
Key Features
Concentration: 1 U/μL
Optimal temperature: 72°C
5′→3′ polymerase activity
Extension speed: ~60 nt/s (1 kb/min)
3′-A overhang, compatible with TA cloning
Required cofactor: Mg²⁺ (2-4 mM optimal)
No proofreading (lacks 3′→5′ exonuclease)
Room temperature stable for shipping
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Product Description
AuTaq DNA Polymerase is a thermostable enzyme derived from Thermus aquaticus. This enzyme has been fundamental in the development and widespread adoption of the Polymerase Chain Reaction (PCR) technique.
AuTaq DNA Polymerase is produced recombinantly in Escherichia coli cells transformed with the cloned pol gene from Thermus aquaticus strain YT-1. The enzyme is purified to high homogeneity and tested for absence of contaminating nucleases and DNA.
Concentration: 1 U/μL
Key Advantages
- Robust performance: Reliable amplification across diverse templates
- TA cloning compatible: 3′-A overhangs facilitate direct cloning
- Cost-effective: Economical choice for routine PCR applications
- Room temperature stable: Enhanced stability for shipping and handling
Physical Properties
Enzymatic Properties
Extension Speed
~60 nucleotides/second at 72°C (1 kb/min)
Optimal Temperature
72°C
Required Cofactor
Mg²⁺ (2-4 mM optimal)